Blar i forfatter "Sjøttem, Eva"

Viser treff 1-15 av 15

    • CALCOCO1 acts with VAMP ‐associated proteins to mediate ER ‐phagy 

      Nthiga, Thaddaeus Mutugi; Shrestha, Birendra Kumar; Sjøttem, Eva; Bruun, Jack-Ansgar; Larsen, Kenneth Bowitz; Bhujabal, Zambarlal; Lamark, Trond; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-11)
      The endoplasmic reticulum (ER) plays important roles in protein synthesis and folding, and calcium storage. The volume of the ER and expression of its resident proteins are increased in response to nutrient stress. ER‐phagy, a selective form of autophagy, is involved in the degradation of the excess components of the ER to restore homeostasis. Six ER‐resident proteins have been identified as ER‐phagy ...

    • The FMRpolyGlycine protein mediates aggregate formation and toxicity independent of the CGG mRNA hairpin in a cellular model for FXTAS 

      Hoem, Gry; Larsen, Kenneth Bowitz; Øvervatn, Aud Karin; Brech, Andreas; Lamark, Trond; Sjøttem, Eva; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-03-28)
      Fragile X-associated tremor/ataxia syndrome (FXTAS) is a neurodegenerative disorder caused by a CGG-repeat expansion in the 5′ UTR of the FMR1 gene on the X-chromosome. Both elevated levels of the expanded FMR1 mRNA and aberrant expression of a polyglycine protein (FMRpolyG) from the CGG-repeat region are hypothesized to trigger the pathogenesis of FXTAS. While increased expression of FMRpolyG leads ...

    • Generation of a PAX6 knockout glioblastoma cell line with changes in cell cycle distribution and sensitivity to oxidative stress 

      Hegge, Beate; Sjøttem, Eva; Mikkola, Ingvild (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-05-02)
      <i><p>Background</i>: The transcription factor PAX6 is expressed in various cancers. In anaplastic astrocytic glioma, PAX6 expression is inversely related to tumor grade, resulting in low PAX6 expression in Glioblastoma, the highest-grade astrocytic glioma. The aim of the present study was to develop a PAX6 knock out cell line as a tool for molecular studies of the roles PAX6 have in attenuating ...

    • Generation of the short TRIM32 isoform is regulated by Lys 247 acetylation and a PEST sequence 

      Garcia-Garcia, Juncal; Overå, Katrine Stange; Khan, Waqas; Sjøttem, Eva (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-05-17)
      TRIM32 is an E3 ligase implicated in diverse biological pathways and pathologies such as muscular dystrophy and cancer. TRIM32 are expressed both as full-length proteins, and as a truncated protein. The mechanisms for regulating these isoforms are poorly understood. Here we identify a PEST sequence in TRIM32 located in the unstructured region between the RING-BBox-CoiledCoil domains and the NHL ...

    • Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs 

      Birgisdottir, Åsa Birna; Mouilleron, Stephane; Bhujabal, Zambarlal; Wirth, Martina; Sjøttem, Eva; Evjen, Gry; Zhang, Wenxin; Lee, Rebecca; O'Reilly, Nicola; Tooze, Sharon A; Lamark, Trond; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-03-04)
      Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1). The latter contains PIK3C3/VPS34, PIK3R4/VPS15, BECN1/Beclin 1 ...

    • NIMA-related kinase 9–mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1 

      Shrestha, Birendra Kumar; Rasmussen, Mads Skytte; Abudu, Yakubu Princely; Bruun, Jack-Ansgar; Larsen, Kenneth Bowitz; Alemu, Endalkachew Ashenafi; Sjøttem, Eva; Lamark, Trond; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-12-19)
      Human ATG8 family proteins (ATG8s) are active in all steps of the macroautophagy pathway, and their lipidation is essential for autophagosome formation. Lipidated ATG8s anchored to the outer surface of the phagophore serve as scaffolds for binding of other core autophagy proteins and various effector proteins involved in trafficking or fusion events, whereas those at the inner surface are needed for ...

    • P62/sequestosome-1, autophagy-related gene 8, and autophagy in Drosophila are regulated by nuclear factor erythroid 2-related factor 2(NRF2), independent of transcription factor TFEB 

      Jain, Ashish; Rusten, Tor Erik; Katheder, Nadja Sandra; Elvenes, Julianne; Bruun, Jack-Ansgar; Sjøttem, Eva; Lamark, Trond; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2015-04-30)
      The selective autophagy receptor p62/sequestosome 1 (SQSTM1) interacts directly with LC3 and is involved in oxidative stress signaling in two ways in mammals. First, p62 is transcriptionally induced upon oxidative stress by the NF-E2-related factor 2 (NRF2) by direct binding to an antioxidant response element in the p62 promoter. Second, p62 accumulation, occurring when autophagy is impaired, leads ...

    • Pax6 Represses Androgen Receptor-Mediated Transactivation by Inhibiting Recruitment of the Coactivator SPBP 

      Elvenes, Julianne; Thomassen, Ernst Ivan; Johnsen, Sylvia Sagen; Kaino, Katrine; Sjøttem, Eva; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)
      The androgen receptor (AR) has a central role in development and maintenance of the male reproductive system and in the etiology of prostate cancer. The transcription factor Pax6 has recently been reported to act as a repressor of AR and to be hypermethylated in prostate cancer cells. SPBP is a transcriptional regulator that previously has been shown to enhance the activity of Pax6. In this study ...

    • A Phylogenetic Study of SPBP and RAI1: Evolutionary Conservation of Chromatin Binding Modules 

      Darvekar, Sagar; Rekdal, Cecilie; Johansen, Terje; Sjøttem, Eva (Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
      Our genome is assembled into and array of highly dynamic nucleosome structures allowing spatial and temporal access to DNA. The nucleosomes are subject to a wide array of post-translational modifications, altering the DNAhistone interaction and serving as docking sites for proteins exhibiting effector or “reader” modules. The nuclear proteins SPBP and RAI1 are composed of several putative “reader” ...

    • The recent transfer of a homing endonuclease gene 

      Haugen, Peik; Wikmark, Odd-Gunnar; Vader, Anna; Coucheron, Dag H.; Sjøttem, Eva; Johansen, Steinar D. (Journal article; Tidsskriftartikkel; Peer reviewed, 2005)
      The myxomycete Didymium iridis (isolate Panama 2) contains a mobile group I intron named Dir.S956-1 after position 956 in the nuclear small subunit (SSU) rRNA gene. The intron is efficiently spread through homing by the intron-encoded homing endonuclease I-DirI. Homing endonuclease genes (HEGs) usually spread with their associated introns as a unit, but infrequently also spread independent of ...

    • SPBP is a sulforaphane induced transcriptional coactivator of NRF2 regulating expression of the autophagy receptor p62/SQSTM1 

      Darvekar, Sagar; Elvenes, Julianne; Brenne, Hanne; Johansen, Terje; Sjøttem, Eva (Journal article; Tidsskriftartikkel; Peer reviewed, 2014)

    • Transforming growth factor-beta-inducible early response gene 1 is a novel substrate for atypical protein kinase Cs 

      Alemu, Endalkachew; Sjøttem, Eva; Outzen, heidi; Larsen, Kenneth Bowitz; Holm, turid; Bjørkøy, Geir; Johansen, Terje (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)
      The protein kinase C (PKC) family of serine/threonine kinases consists of ten different isoforms grouped into three subfamilies, denoted classical, novel and atypical PKCs (aPKCs). The aPKCs, PKC?/? and PKC? serve important roles during development and in processes subverted in cancer such as cell and tissue polarity, cell proliferation, differentiation and apoptosis. In an effort to identify novel ...

    • TRIM27 is an autophagy substrate facilitating mitochondria clustering and mitophagy via phosphorylated TBK1 

      Garcia, Juncal Garcia; Berge, Anne Kristin McLaren; Overå, Katrine Stange; Larsen, Kenneth Bowitz; Bhujabal, Zambarlal Babanrao; Brech, Andreas; Abudu, Yakubu Princely; Lamark, Trond; Johansen, Terje; Sjøttem, Eva (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-09-16)
      Tripartite motif-containing protein 27 (TRIM27/also called RFP) is a multifunctional ubiquitin E3 ligase involved in numerous cellular functions, such as proliferation, apoptosis, regulation of the NF-kB pathway, endosomal recycling and the innate immune response. TRIM27 interacts directly with TANK-binding kinase 1 (TBK1) and regulates its stability. TBK1 in complex with autophagy receptors is ...

    • TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1 

      Overå, Katrine Stange; Garcia-Garcia, Juncal; Bhujabal, Zambarlal; Jain, Ashish; Larsen, Kenneth Bowitz; Deretic, Vojo; Johansen, Terje; Lamark, Trond; Sjøttem, Eva (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-12-02)
      The tripartite motif (TRIM) proteins constitute a family of ubiquitin E3 ligases involved in a multitude of cellular processes, including protein homeostasis and autophagy. TRIM32 is characterized by six protein–protein interaction domains termed NHL, various point mutations in which are associated with limb-girdle-muscular dystrophy 2H (LGMD2H). Here, we show that TRIM32 is an autophagy substrate. ...

    • The ubiquitin E3 ligase TRIM27 emerges as a new player in mitophagy 

      Berge, Anne Kristin McLaren; Garcia, Juncal Garcia; Sjøttem, Eva; Olsvik, Hallvard Lauritz (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-01-05)
      Mitochondria are the center for energy production, cell fate determination and synthesis of essential biomolecules in cells. Hence, mitochondrial quality control mechanisms are essential for cellular health. Failure of these control mechanisms may lead to damaged mitochondria that represent a threat to cell survival. Mitophagy is a selective autophagy process that removes damaged mitochondria ...